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STEMCELL Technologies Inc cd3+ immunomagnetic negative selection #17951
Cd3+ Immunomagnetic Negative Selection #17951, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immunomagnetic+negative+selection+cd3/immunomagnetic+negative+selection+19853/pm39632825-103-23-28
Average 90 stars, based on 1 article reviews
cd3+ immunomagnetic negative selection #17951 - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Chromatin-based, in cis and in trans regulatory rewiring underpins distinct oncogenic transcriptomes in multiple myeloma
Article Snippet: Normal donor BM mononuclear cells from BM aspirates (BM-MNCs) were isolated by Ficoll (Histopaque, Sigma). .. The BM-MNCs were pre-cleared of T cells and Monocytes by consecutive immunomagnetic negative selection (CD3 and CD14-EasySep StemCell Technologies) following the manufacturer’s instructions. .. The samples were stained (1:100 antibody dilution) and sorted for CD138+, CD319+, CD27+, CD45+ and CD38+ (positive), for CD2−, CD3−, CD14−, CD16−, GPA− and 7AAD- (negative) and CD19+/− (positive or negative, Supplementary Table and Supplementary Fig. ) (FACSAriaII, BD Biosciences).



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A Immunohistochemical staining of <t>CD3,</t> CD4, CD8, CD68 and Ki-67 in CRC tissues showed the presence of T-cells populations (CD4 + , CD8 + ) and macrophages (CD68 + ) in MSI and MSS patients. Ki-67 was used as a control proliferation marker of tumor cells; ( B ) Quantification of positive cells/area was performed with QuPath Software. Significance shown refers to Wilcoxon test p -values *< 0.05, **< 0.01, ***< 0.001.
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A Immunohistochemical staining of CD3, CD4, CD8, CD68 and Ki-67 in CRC tissues showed the presence of T-cells populations (CD4 + , CD8 + ) and macrophages (CD68 + ) in MSI and MSS patients. Ki-67 was used as a control proliferation marker of tumor cells; ( B ) Quantification of positive cells/area was performed with QuPath Software. Significance shown refers to Wilcoxon test p -values *< 0.05, **< 0.01, ***< 0.001.

Journal: Cell Death & Disease

Article Title: Colorectal cancer patients-derived immunity-organoid platform unveils cancer-specific tissue markers associated with immunotherapy resistance

doi: 10.1038/s41419-024-07266-5

Figure Lengend Snippet: A Immunohistochemical staining of CD3, CD4, CD8, CD68 and Ki-67 in CRC tissues showed the presence of T-cells populations (CD4 + , CD8 + ) and macrophages (CD68 + ) in MSI and MSS patients. Ki-67 was used as a control proliferation marker of tumor cells; ( B ) Quantification of positive cells/area was performed with QuPath Software. Significance shown refers to Wilcoxon test p -values *< 0.05, **< 0.01, ***< 0.001.

Article Snippet: T-cells and Myeloid Derived Suppressor Cells (MDSCs) were isolated from patient peripheral blood mononuclear cells (PBMCs) by CD3 + immunomagnetic negative selection (#17951, StemCell Technologies) and CD33 + immunomagnetic positive selection (#17876, StemCell Technologies) following positive selection with CD3 + immunomagnetic positive selection (#17851, StemCell Technologies), respectively.

Techniques: Immunohistochemical staining, Staining, Control, Marker, Software